Sanger Sequencing helps in the identification of the base at the end portion of a specific fragment of DNA. Sanger performs this step by removal of an oxygen atom specifically from the ribonucleotide. This particular nucleotide is known as the dideoxynucleotide. So, this is how this process takes place. One important thing that you should know about is that both Sanger Sequencing Services and PCR make use of similar starting materials.